The clinical importance of color Doppler ultrasonography in puncture related complications of hemodialysis vascular access
Egyptian Journal of Radiology and Nuclear Medicine volume 50, Article number: 89 (2019)
The long-term survival of patients on hemodialysis (HD) is dependent on adequate HD which depends on quality and degree of functionality of vascular access. The aim of our study is to detect the role of color Doppler ultrasonography in evaluation of arteriovenous fistula (AVF).
Materials and methods
Between January 2017 and October 2017, 60 patients had complicated AVF at Qena General Hospital & Qena University Hospital as primary and tertiary centers respectively with the same age group between 18 and 50 years old, had no diabetes mellitus were evaluated using color duplex-Doppler ultrasound (CDUS) after AVF maturation for detection of complications and comparison between primary and tertiary centers of its incidence.
The overall results revealed that 11 patients from 30 patients that had been referred to tertiary centers have complicated AVF (36.7%), and 19 patients had no shunt complication (63.3%). Twenty-two patients from 30 patients that had been referred to primary HD centers had complicated shunts. Thrombosis at tertiary centers represents 13.3%, stenosis 3.3% (1 patient), aneurysmal formation had 6.6% for (2 patients), hematoma 6.6%(2 patients), infection 3.3% (1 patient), venous hypertension 3.3% (1 patient), thrombosis and hematoma at primary centers 16.6% (5 patients for each), aneurysmal formation and infection 13.3% for each, and stenosis 10%.
CDUS is a readily-available, non-invasive, risk-free, bed-side technique that allows precise anatomic knowledge, qualitative, and quantitative data of the upper limb vascular system which greatly help in preoperative planning of AVF creation, assessment of prime time for puncture (maturation), early detection of complications, and choice of appropriate therapeutic procedure for their treatment.
Hemodialysis has an important role in long-term survival of patients with end-stage-renal disease (ESRD). To maintain them on long-term dialysis, vascular access procedures are required . A well-functioning vascular access has a good blood flow, excellent patency, and allows easy and repetitive cannulation with two needles. The arteriovenous fistula (AVF) provides the best access for longevity and lowest morbidity and mortality; however, arteriovenous grafts remain clinically important in elderly patients and whom AVFs are not feasible . After surgical creation, the vein distended to become a successful arteriovenous fistula (AV). Fistula undergoes a remodeling process that is referred to as maturation. Although somewhat variable, these changes occur relatively rapidly, resulting in a fistula that can be repetitively used and which can provide adequate dialysis treatments . Ideal time needed for arteriovenous fistula (AVF) maturation is still controversial. Early detection of access dysfunction, complication allows for subsequent interventions that help to reduce the rate of access failure . Failure of the A-V fistula can be early or late. Early failure of the vascular access for hemodialysis is accounted for the period between creation and the beginning of the work of the fistula, or within the first 3 months of its use. On the other hand, late failure of the A-V fistula is considered if it happens after 3 months of its use for hemodialysis . US can help also to identify other complications associated with fistulas including abscess, aneurysm, steal syndrome, and hematoma scan also be evaluated by color Doppler examination . Color Doppler ultrasonography enables preoperative vascular mapping for AVF creation, assessment of prime time for puncture, early detection of complications, and choice of appropriate therapeutic procedures for correction . The long-term survival and quality of life of patients with ESRD on hemodialysis is dependent on the adequacy of dialysis via an appropriately placed vascular access . Duplex ultrasound is rapidly emerging as an essential investigation, and its widespread introduction in the future will have major resource implications .
The current study is observational prospective randomized controlled trial study of 60 patients of end stage renal disease (ESRD) an observational prospective study conducted at Qena University Hospital during the study period from January 2017 to June 2018.
The study included 60 patients with ESRD for AVF evaluation, aged from 18 to 50 years.
Patients on hemodialysis with late complication of AVF
Age group from 18 to 50 years old
All AVFs had been done at Qena University Hospital with the same preoperative assessment and operative technique
Those with early complications
Patients with peripheral vascular disease
Equipment and supplies
Grey scale and color Doppler GE LOGIC P7 ultrasound machine with high frequency transducers (8–10 MHz). Standard supplies for ultrasonic exam: acoustic coupling gel, gloves, and skin wipes
The preoperative criteria to be met for vascular access are summarized as follows:
Arteries 2.0 mm or larger
Veins, 2.5 mm, or larger for AVF
Depth of the vein from the skin surface
< 5 mm
Superficial vein continuing to the deep venous system
Absence of central venous stenosis
Absence of evidence of arterial stenosis segments
Patients who undergo upper limb AVFs will be examined with US Doppler after 3 months, 6 months, and 12 month for detection of late complications.
Indications for CDUS examination 
Clinical symptoms and signs of insufficiency of AV access:
Poor arterial flow with access collapse
Ischemia of the distal limb
-Impaired matured fistula
Clinical suspicion of infection
Perigraft aneurysm or pseudo-aneurysm or mass
Malfunction of the fistula as evidenced by:
Increased venous pressure more than 200 mmHg on a 300 cc/min pump
Increased recirculation time ≥ 15%
Decreased reduction rate of urea ˂ 60%
Post-procedure ultrasound technique using step-by-step protocol for vascular access assessments 
The following will be assessed:
The diameter and compressibility of the draining vein is studied routinely in the caudal, mid-, and cranial portions of the forearm, and similarly in the upper arm.
The depth of the anterior wall of the AVF from the skin surface is also measured.
Blood flow is measured in the AVF in ml/min using the volume flow measurement function of the duplex instrument at a site far enough from the anastomosis. Three measurements will be performed, and the final value used in the study will be the arithmetic mean.
Assessment of AVF complications: sites of examination included
The afferent artery
Site of anastomosis
The draining veins as far as the subclavian vein
The arterial tree distal to the AVF in cases suffering from steal syndrome
Doppler parameters and measurements
Each of the following parameters was measured at the site of AV anastomosis, at the afferent artery and finally at the draining vein.
Volume of blood flow (ml/min)
The distal arterial tree was examined in cases suffering from steal syndrome.
In this study, we started with 60 patients with ESRD candidate for hemodialysis AVF whom have no history of diabetes mellitus, and various types of well-functioning AVFs had been done for them at Vascular Surgery Department-South Valley University Hospitals . 24 patients (40%) had B-C, (18 patients,30%) had B-B fistula (Fig. 1) and (18 patients, 30%) had R-C fistula. Fifty percent of patient populations had been referred to the Hemodialysis Unit of SVU Hospitals as a tertiary hemodialysis center where good nursing, and the other 50% of patient population had been referred to Qena General Hospital as a primary hemodialysis center.
Maturation of an AV fistula typically occurs within the first 4 weeks after creation. The mean age of maturation in our study of the fistulae encountered was 4 ± 0.379 weeks.
We found that the mean blood flow rate in adequately functioning AVFs was 816.21 ± 152 ml/min. after creation of fistula.
Our study demonstrates that 22 (73.3%) out of 30 patients who referred from the primary center developed AVF complications as follow: hematoma [5 cases (16.6%)] with same frequency regarding thrombosis, infection [4 cases (13.3%)], stenosis and peudo-aneurysm with the same frequency [3 cases (10%)], venous aneurysm and venous hypertension also having the same frequency [1 case (3.3%)]. While, 11 patients from 30 patients had been referred to tertiary center who have complicated AVF (36.7%), and 19 patients had no shunt complication (63.3%). Thrombosis 13.3% (4 patients), hematoma 6.6% (2 patients), aneurysmal formation had 6.6% for (2 patients) one patient on the venous side and the other on the arterial side, stenosis 3.3% (1 patient), infection 3.3% (1 patient), and venous hypertension 3.3% (1 patient) (Table 1 and Figs. 2, 3, 4, 5, 6, 7).
Vascular access problems remain the vulnerable point of modern HD . For evaluation of access dysfunction, the initial, most practical, and cost-effective method is physical examination . US confirm the results of physical examination such as inflow stenosis and outflow stenosis. Moreover, it provides important information about the functional severity like brachial artery flow rates [14, 15]. By combining the findings of US and physical examination, the treatment methods can be determined, such as angioplasty, revision surgery, or conservative management . Moreover, Doppler US shortens angioplasty time, as it gives information on the stenosis site . Thus, by the use of US, more clinical needs are satisfied .
The present study demonstrated that, during the first 11 months of newly created AVFs, a great percentage of patients encounter cannulation related complications were significantly higher in primary than tertiary centers of hemodialysis.
In this study, we evaluated the clinical utility of CDUS for early detection of complications in AV dialysis access. We found that the number of female patients exceeded that of males, with 36 female patients and 24 male patients, representing 60 and 40% of all patients, respectively. This correlates with the findings of the previous studies who found that fistulas are less likely to be usable for dialysis in female than in male patients .
Among our study population, the most common shunt complication associated with HD was access to thrombosis (n = 5, 16.6% in primary centers and n = 4, 13.3% in tertiary centers). Thrombosis is usually located in the arteriovenous anastomosis of the AVF. In our study, thrombosis was detected at the venous side of the fistula in most cases; diagnosis of thrombosis was established by absence of flow using color or pulsed Doppler together with hypoechoic or echogenic thrombus filling the lumen.
Stenosis of HD vascular access is common and may lead to thrombosis and the loss of the access. Thus, detection of stenosis in AVF before thrombosis could offer a strategy to improve AVF survival by early intervention . Stenosis in AVF develops more frequently in juxta-anastomotic location, up to 4 cm from the anastomosis . Among 4 patients diagnosed with stenosis, one cases had post-fistula stenosis, three cases had stenosis at the anastomotic site. This was in accordance with the finding of Tirinescu et al. , who performed a large observational study on 97 patients, and reported juxta-anastomotic localization of stenosis in most cases, in the forearm, and in the upper arm AVF equally.
Clinically pseudoaneurysms are diagnosed by the presence of a pulsatile mass with systolic murmur. However, CDUS is particularly suitable to determine the extent of the aneurysm and to demonstrate thrombotic material within the aneurysmatic sac . Out of the examined cases in our study, 6 patients had pseudoaneurysm (12%), 4 at the venous side of the AVF, 1 at the site of anastomosis, and 1 at the arterial side, which is relatively rare. Puncture of an AVF either as part of standard dialysis needling or from intervention can result in prolonged bleeding and pseudoaneurysm formation. However, the mechanism of formation of true aneurysms in AVFs is less clear. This is may be attributed to also to repeated needling with consequent development of multiple small fibrous scars in the vessel wall that expand with time and result in localized aneurysmal areas [22, 23].
Tenderness and erythema along the access can indicate infection. An untreated access infection may lead to bacteremia, sepsis, hemorrhage, and, if left untreated, possible death. In this study, 4 patients 13.3% in primary centers had an infected AVF, and only one patient had an infected AVF 3.3% in tertiary centers, due to the use of complete antiseptic measures in tertiary centers.
Venous hypertension results from arterialization of the venous system proximal to an arteriovenous fistula with central venous occlusion . Our study included 2 patients with venous hypertension (4%) due to central venous stenosis.
The CDUS findings in this series were helpful to determine further therapeutic management in patients with AV dysfunction.
The limitation of this study is the relatively small number of patients with selection bias including only patients with suspected AV dysfunction on physical examination. A larger study including all patients for surveillance of AVF is recommended.
CDUS is a noninvasive diagnostic tool for early detection of complications of AVFs that allows monitoring of the AVF blood flow and detection of possible causes of vascular access malfunction. Owing to its low cost and availability, it should be used as the first-line imaging modality for dysfunctional AVF. Well-qualified nurses are very important in reduction of AVF complications, and tertiary centers should help primary centers in continuous education of nurses.
Availability of data and materials
The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.
Color Doppler ultrasonography
End diastolic velocity
End stage renal disease
Peak systolic velocity
South Valley University
Asif A, Roy-Chaudhury P, Beathard GA (2017) Early AVF failure: a logical proposal for when and how to intervene. C J Am Soc Nephrol. 1(2):332–339
Cho S, Lee YJ, Kim SR (2017) Clinical experience with ultrasound guided angioplasty for vascular access. Kidney Res Clin Pract 36:79–85
Davidson I, Chan D, Dolmatch B, Hasan M, Nichols D, Saxen R (2008) Duplex ultrasound evaluation for dialysis access selection and maintenance: a practical guide. J Vascular Access. 9(1):1–9
Ethier J, Mendelssohn DC, Elder SJ et al (2008) Vascular access use and outcomes: an international perspective from the Dialysis Outcomes and Practice Patterns Study. Nephrology Dialysis Transplantation. 23:3219–3226
Ferring M, Henderson J, Wilmink A, Smith S (2008) Vascular ultrasound for the pre-operative evaluation prior toAVF formation for hemodialysis: review of the evidence. Nephrol Dial Transplant. 23(6):1809–1815
Gerald A, Beathard MD (2016) PhD, Maturation and evaluation of the newly created hemodialysisarteriovenous fistula. Sep 12
Guedes Marques M, Ibeas J, Botelho C, Maia P, Ponce P (2015) Doppler ultrasound: a powerful tool for vascular access surveillance. Semin Dial 28:206–210
Kumbar L, Karim J, Besarab A (2012) Surveillance and monitoring of dialysis access. Int J Nephrol 2012:649735
Lazarides MK, Georgiadis GS, Argyriou C (2014) Aneurysm formation and infection in AV prosthesis. J Vasc Access 15(Suppl 7):S120–S124
Lockhart ME (2001) Robbin ML: hemodialysis access ultrasound. Ultrasound Q. 17:157–167
Malovrh M (2008) Nativearteriovenous fistula: preoperative evaluation. Am J Kidney Dis 39:1218–1225
Matsui S, Nakai K, Taniguchi T et al (2012) Systematic evaluation of vascular access by color-Doppler ultrasound decreased the incidence of emergent vascular access intervention intervention therapy and X-ray exposure time: a single-center observational study. Ther Apher Dial 16:169–172
Mudoni A, Cornacchiari M, Gallieni M, Guastoni C, McGrogan D, Logias F (2015) Aneurysms and pseudoaneurysms in dialysis access. Clin Kidney J 8:363–367
Nalesso F, Garzotto F, Petrucci I, Samoni S, Virzì GM, Gregori D, Meola M, Ronco C (2018) Standardized protocol for hemodialysis vascular access assessment: the role of ultrasound and color Doppler. Blood Purif. 45:260–269
National Kidney Foundation. K/DOQI Clinical Practice Guidelines for Vascular Access: update 2000.Am J Kidney Dis. 2001;37(1):137–81.
Salman L, Beathard G (2013) Interventional nephrology: physical examination as a tool for surveillance for the hemodialysis arteriovenous access. Clin J Am Soc Nephrol 8:1220–1227
Shenoy S, Darcy M (2013) Ultrasound as a tool for preoperative planning, monitoring, and interventions in dialysis arteriovenous access. AJR 201:W539–W543
Soliman H, Raafat T, Abdelhamid YM (2015) Angiographic mapping of AV fistula related vascular complications in ESRD via multislice CT; adjuvant role in correlation with CDUS. EJRNM 46:665–674
Tirinescu DC, Bondor CJ, Vlăduțiu DŞ, Pațiu IM, Moldovan D, Orășan R, Kacsó IM (2016) Ultrasonographic diagnosis of stenosis of native arteriovenous fistulas in haemodialysis patients. Med Ultrason 18:332–338
Wiese P, Nonnast-Daniel B. Colour Doppler ultrasound in dialysis access. Nephrol Dial Transplant. 2004;19(8):1956–63.
Vascular access 2006 work group (2006) Clinical practice guidelines for vascular access. Am J Kidney Dis 48(Suppl 1):S176–S247
Zamboli P, Fiorini F, D’Amelio A, Fatuzzo P, Granata A (2014) Color Doppler ultrasound and arteriovenous fistulas for hemodialysis. J Ultrasound 17:253–263
Minion DJ, Moore E, Endean E (2005): Revision using distal inflow: A novel approach to dialysis-associated steal syndrome. Ann Vasc Surg; 19(5): 625–8.
This study had no funding from any resource.
Ethics approval and consent to participate
The current study has been approved by the Ethics committee of the Faculty of Medicine, South Valley University, Qena, Egypt, and was done in accordance with Declaration of Helsinki. An informed written consent has been obtained from every included patient.
Ethics committee’s reference number: 12/2016.
Consent for publication
All patients included in this research gave written informed consent to publish the data contained within this study. If the patient was less than 16 years old, deceased, or unconscious when consent for publication was requested, written informed consent for the publication of this data was given by their parent or legal guardian.
The authors declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.
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Hassan, M.H., Abdelrazek, G.M. & Hashim, A.A. The clinical importance of color Doppler ultrasonography in puncture related complications of hemodialysis vascular access. Egypt J Radiol Nucl Med 50, 89 (2019). https://doi.org/10.1186/s43055-019-0087-7