The extent of CC malformation varies from partial to complete agenesis or just hypoplasia; its thickness decreases along all its parts. In partial agenesis, the posterior part is not developed. However, cases of anterior agenesis have also been reported . In CCA, identification of indirect signs of CCA in the axial planes of scanning allows suspecting this pathology. In order to make the final diagnosis, it is necessary to assess CC in the middle sagittal plane . Fetal MRI allows better direct visualization of the CC. Moreover, the increased contrast resolution of fetal MRI allows detection of subtle CNS abnormalities that are prognostic indicators for the postnatal neurodevelopmental outcome . Many disciplines, including obstetric, perinatology, genetics, pediatric surgery, and neurology, are involved in fetal MRI; therefore, the future of fetal MRI can be achieved through multidisciplinary collaborative team .
A total of 27 pregnant females participated in this study according to the inclusion criteria suspected to have CCA on prenatal US. The maternal age ranged from 17 to 41 years with a mean 29.5 years (SD = ± 5.97). The complete development of CC is around 18 to 19 GW when it is detected in prenatal imaging . Therefore, in this study the GA ranged from 20 to 37 WG; with a mean 31 WG and 4 days (SD± 5.2 WG). Concerning the maternal obstetric history, most of the pregnant females, 20 cases, were multi-gravida (74%), while only 7 cases (26%) were primi-gravida. Regarding their consanguinity, only 9 (33.3%) had positive consanguinity, while the majority 18 cases (66.7%) were not relatives. Concerning the previous history of fetal CNS anomalies; only 5 (18.5%) gave history of previous CNS anomalies in their offsprings. Regarding the fetal gender, the majority were males, 19 fetuses (70.3%) while 8 fetuses (29.7 %) were females; these were coinciding with a study conducted by Manganaro et al. : they revealed that the average maternal age was 32.7 years (SD = ± 5.8), male fetuses were more numerous than female fetuses. Considering the fate of pregnancy, Manganaro et al.  found that 27% were terminated; 5.8% were stillbirths; and 22% were born. In the current study, 5 cases (18.5%) underwent termination of pregnancy (abortion), 2 cases (7.5%) had intra-uterine fetal death, while the majority 20 cases (74%) continued their pregnancy to 37th WG.
Out of 27 fetuses who were suspected to have CCA on prenatal US, fetal MRI diagnosis agreed with prenatal US in 25/27 fetuses (92.5%); they had CCA, while there were disagreements in 2/27 (7.5%) FP on prenatal US; they had normal CC by pre- and postnatal MRI. A study conducted by Sakar et al.  suspected 21% CC abnormality by US. MRI confirmed in about 83% of cases while in rest (16.7%), it disproved US diagnosis.
In the current study, CCA were classified into either complete 77.7% (n = 21) or partial agenesis 14.8% (n = 4), and no hypoplastic cases. This was in accordance with Rüland et al. ; they diagnosed complete agenesis in 76 %, partial agenesis 14 %, and a hypoplastic CC in 9 % of cases.
The classification of CCA can be either an isolated anomaly or associated with other abnormalities: neurological or non- neurological anomalies. This study observed that only 5/27 (18.5%) had isolated CCA, while the majority 20/27 (74%) were complex form and associated with other anomalies. This was concordance with a study done by Manganaro et al. ; they revealed that fetal MRI demonstrated isolated CCA in 26.9%, while the majority of the cohort about 73.1% CCA was associated with other anomalies. Another study done by Rüland et al.  that found about 29 % was isolated and 71 % was non-isolated form. However, these results are higher than what had been reported in a previous study done by Tang et al. ; they found that only 7% of cases of CCA were isolated by fetal MRI; this may be due to the younger GA and increased number of fetuses in their study.
Ventriculomegaly was not considered an additional finding because dilatation of the atria of the lateral ventricle (more than 10 mm) can be attributed to callosal agenesis; it is the main feature of isolated CCA. In this study, there were 19/27 cases (70.4%) that had ventriculomegaly; one of them shows abnormal morphology of the lateral ventricle involving the posterior horn, which is incompletely formed, being deformed in shape, with undulations along its margin, and focally dilated due to communicating with porencephalic cyst.
For cases diagnosed to have complex form of CCA, they are classified into: most of them 14 cases (70%) associated with other neurological anomalies, while only 4 cases (20%) associated with other non-neurological anomalies, and the last 2 cases (10%) had both anomalies.
The most common associated neurological finding was IHC about 6/16 cases (37.5%), 5 cases (31.3%) had NTD, 5/16 cases (31.3%) of cerebellar hemispheres and vermian hypoplasia, 3 cases (18.8%) had tonsillar herniation, 3 cases (18.8%) had delayed and abnormal cerebral sulcation, 1 case (6.3%) had periventricular GMH, 1 case with microcephalic skull (6.3%), and 1 case brain stem hypoplasia (6.3%). The sensitivity of fetal MRI compared to prenatal US in detection of neurological abnormalities was 100%, while 2D US was 75%. In a study conducted by Tang et al. , fetal MRI identified abnormalities not detected by prenatal sonography in most (83%) patients; this difference is higher than that detected with current study in which fetal MRI findings coincided with that observed by prenatal US in 12 cases (75%). However, MRI added additional information to the US findings in 4 cases only (25%) in the form of: 1 case with small dorsal IHC, vermian hypoplasia, and small occipital meningocele; 2 cases with tonsillar descent (1 case was Chiari I associated with frontal encephalocele, abnormal sulcation, and hemimegalencephaly), the other case with small sacral meningocele (Chiari II).
A study done by Manganaro et al.  found that 39.5% of cases had cortical developmental defects by fetal MRI. In another study conducted by Tang et al. , they observed that 42.8% of cases had abnormal sulcal morphology, with gyral malformations. However, in the current study, only 3 cases (18.8%) had delayed and abnormal cerebral sulcation. Therefore, more sensitive methods such as 3D morphometric study, diffusion-weighted, and diffusion tensor imaging might detect other sulcal abnormality cases.
Posterior fossa abnormalities were also a common additional finding, in the form of 5/16 cases (31.3%) of cerebellar and/or vermian hypoplasia, 3 cases (18.8%) of tonsillar herniation, and 2 cases (12.5%) of occipital meningocele/encephalocele. This was in agreement with a study done by Manganaro et al. ; they confirmed that fetal MRI detected associated cerebellar malformations in 30%. They also revealed that fetal MRI-diagnosed CCA with cerebellar hemispheric abnormalities were more common than vermian abnormalities; they stated that it may be due to the difficulty in evaluating the fetal vermis or due to termination of those cases with prenatally diagnosed cerebellar abnormalities. However, in current study, fetal MRI diagnosed CCA with both cerebellar hemispheric abnormalities and vermian abnormalities equally.
In the current study, the most common finding was IHC, 6/16 cases (37.5%), while the least association was periventricular GMH and a microcephalic skull each represented in 1 case only (6.3%). In a study conducted by Manganaro et al. , they detected that about 19.7% had IHCs and only 9.2% had microcephaly.
Regarding the associated non-neurological anomalies, Manganaro et al.  found that 22.4% had thoracoabdominal, 18.4% had musculoskeletal, 17% had craniofacial, and 14.5% had cardiac malformations. However, in this study, they were identified equally by prenatal US and MRI in 5 cases. In the last case that had both neurological and non-neurological anomalies, MRI added additional findings in the diagnosis of associated non-neurological anomalies (hypoplastic lung and bilateral small eye globes) which were missed by prenatal US. The most common non-neurological anomalies were renal abnormalities represented in 2 cases; one case in the form of renal pelvic dilatation while the other was bilateral enlarged multicystic kidneys (Meckel-Gruber syndrome), it also had combined anomalies/associated neurological anomalies, which were enlarged posterior fossa showing large cyst which is communicating with the 4th ventricle, as well as small occipital meningocele, hypoplastic laterally displaced cerebellar hemispheres with absent cerebellar vermis, widely separated occipital horns of the lateral ventricles, 1 case with thick nuchal fold (Down syndrome), 1 case of a duodenal atresia with subsequent polyhydramnios, 1 case of a diaphragmatic hernia, and last case with skeletal abnormalities (bilateral talipes equiovarus and clenched hands). It also had combined anomalies/associated neurological anomalies in the form of large dorsal IHC, also diffusely delayed sulcation pattern, enlarged retrocerebellar space with small cerebellar hemispheres, absent inferior vermis, and hypoplastic brain stem.
Prenatal detection of CCA presents a challenge as the US signs may be very subtle or appear late after the second-trimester scans . Many studies, such as Wagenvoort et al.  and Malinger et al.  concluded that MRI is superior to US, particularly in the diagnosis of callosal anomalies. Raafat et al.  studied the prevalence and value of fetal MRI in the diagnosis of midline cerebral anomalies. They revealed that 5 cases of partial callosal agenesis were missed by US and diagnosed by MRI. The diagnostic accuracy for detecting failed commissuration was 86.49% for US and 100% for MRI. In the current study, upon correlating the fetal MRI findings to the final diagnoses, 24 cases (88.9%) were true positive (TP) and two cases (7.5%) were false positive (FP) by US (7.5%), being true negative (TN) by MRI as they had a normal CC. MRI did not miss the diagnosis in any of the cases false negative (FN = 0). While one case (3.7%) was FP diagnosed as isolated partial CCA, which was having a normal CC in postnatal MRI. The diagnostic performance of fetal MRI in diagnosis of CCA was as follows: sensitivity 100%, specificity 67%, PPV 96%, NPV 100, and accuracy 96.3% to accuracy of 88.8% for prenatal US.
Superior soft tissue contrast, relative operator independence, and a large field of view of fetal MRI make it a valuable adjunctive complementary imaging to prenatal 2D US with high diagnostic performance in fetuses with CCA. However, MRI still has some disadvantages which include fetal motion artifact which affects image quality and its ability to obtain a true sagittal plane, although it is reduced significantly by using the ultrafast MR techniques; poorer spatial resolution than US; high cost; and lack of expertise. MRI showed superior value to US in diagnosis of IHC, vermian abnormalities, tonsillar herniation, NTD, and abnormal cerebral sulcation. While in non-neurological anomalies MRI was superior in detection of hypoplastic lung and small eye globes. The result is not a technical advantage of the imaging method but due to the fact that fetal MRI examination is always done for a specific organ of concern not for whole fetal body; however, prenatal US is used for screening the whole body for fetal anomalies.
This study highlights the diagnostic value of the fetal MRI in the diagnosis of CCA; it has the ability to prognosticate the developmental outcome through detection of associated anomalies and allows informing the family about the neurodevelopmental outcome of the child. Therefore, continuous extended broad researches of fetal MRI of CCA should be done, and longer follow-up is needed because behavioral and cognitive difficulties may not be detected until school age.
The study is limited by the fact that it included only fetuses with prenatal US diagnosis of CCA and excluded the cases with other brain anomalies; also, the study relied on the prenatal US diagnosis; which depend on the judgment of obstetrician whose learning curve was probably variable as US is an operator-dependent study. Furthermore, the readers were from the same institution; therefore, some variability in results if other institutions replicated the work cannot be ruled out. Finally, fetal MRI was performed at many different GA, which is likely affecting the sensitivity of fetal MRI for CCA diagnosis; thus, performing fetal MRI at a consistent GA would probably give a better idea of the accuracy of the study.