Role of diffusion-weighted MRI in characterization of mediastinal masses

Abstract

Background

Diffusion MR imaging (DWI) is a widely available non-invasive non-contrast functional MR imaging technique with short acquisition time. It helps in the analysis of tissue characteristics based on the diffusion of water protons within the tissue. Quantitative assessment of a mass is possible by calculating its apparent diffusion coefficient (ADC) value which is inversely correlated with tissue cellularity. So, DWI has diagnostic potential to distinguish benign from malignant tumors because of the tendency of the latter to show lower ADC values and more restricted diffusion. The aim of our work is to evaluate the use of DWI and ADC value measurement in differentiation between benign and malignant mediastinal tumors.

Results

This study included 44 cases of mediastinal masses: 27 males and 17 females. The mean ADC value of malignant mediastinal lesions was significantly lower than the mean ADC value of benign mediastinal lesions, with mean ADC 1.39 ± 0.26 in benign mediastinal lesions and mean ADC 0.86 ± 0.35 in malignant mediastinal lesions. This study also revealed that the cut-off threshold of ADC value for the differentiation between malignant and benign lesions was 1.11 × 10^-3 mm²/s, with an area under ROC curve of 0.93. The sensitivity and specificity of our cutoff ADC values were 90.9% and 100%, with 100% positive predictive value and 76.9% negative predictive value.

Conclusion

DWI with calculation of ADC value is functional MR imaging technique used in the analysis of tissue characteristics and quantitative assessment of a mediastinal mass. So, it can distinguish benign from malignant tumors because of the tendency of the malignant lesions to show more restricted diffusion and lower ADC values.

Background

The differential diagnosis of mediastinal lesions includes a wide variety of neoplastic, congenital, vascular, and lymphatic etiology [1]. The location and composition of the mediastinal lesions are essential to limit the diagnostic possibilities [2].

Computed tomography (CT) is considered the primary modality of choice for morphological evaluation of mediastinal tumors. Magnetic resonance imaging (MRI) is considered a second-line test. Also, 18F-fluorodeoxyglucose positron emission tomography/CT (18F-FDG-PET/CT) is widely used for assessing cell metabolism and metabolic activity of the lesions pre and post-therapy [3].

Nowadays, the role of MR imaging expands due to its high soft-tissue contrast. It is better than CT in differentiation between cystic and solid masses, identification of cystic and solid components within complex lesions, and distinguishing thymic hyperplasia and normal thymus from thymic neoplasm. Also, MRI can give a better assessment of the relations of the lesion with the pericardium, heart, spinal cord, and vascular structures [2].

With the advances in MR systems, diffusion-weighted imaging (DWI) of the chest has become possible with fast imaging time that minimizes the effect of respiration and cardiac movement. DWI helps in the analysis of tissue characteristics based on the diffusion of water protons within the tissue. Quantitative assessment of a mass is possible by calculating its apparent diffusion coefficient (ADC) value which is inversely correlated with tissue cellularity. So, DWI has diagnostic potential to distinguish benign from malignant tumors because of the tendency of the latter to show lower ADC values and more restricted diffusion [2, 4]. Also, it is useful besides other modalities in differentiating lymphoma from sarcoidosis in mediastinal and hilar lymphadenopathy [5]. Moreover, DWI is used to characterize lung lesions, to predict tumor invasiveness, to detect tumors in collapsed lungs, and for nodal staging of lung cancer [6]. Quantitative analysis of DWI also enables differentiation of lymph nodes with and without metastasis [7]. Potential future applications of DWI in malignancies include monitoring the treatment response after chemotherapy or radiation [6].

The primary objective of our study was to evaluate the potential of DWI and calculated ADC value to characterize mediastinal lesions and to differentiate between benign and malignant tumors. So, it can enhance the non-invasive approaches by which patients can be diagnosed and avoid unnecessary intervention.

Methods

The Institutional Review Board of our Radiology Department approved the design of the study and the use of clinical data. Written consent was obtained from the patients or their parents prior to the procedures.

This study included 44 cases of mediastinal masses: 27 males and 17 females with mean age of 34.8 (range, 1-69 years). All were primarily diagnosed by CT. Cases were referred from oncology and chest departments for MRI assessment between February 2018 and January 2020. The complaints varied between dyspnea, chest pain, cough, hemoptysis, dysphagia, fatigue, and loss of weight. The flow chart of our study is illustrated in Fig. 1.

Fig. 1

Consort flowchart

Inclusion criteria

Patients with mediastinal masses were diagnosed by CT.

Exclusion criteria

General criteria of contra-indication to MRI as patients with pacemaker, cochlear implants, cerebral aneurysm clips, and ocular metallic foreign body.

All patients were subjected to the following:

• Proper clinical evaluation

• Revision of their laboratory investigations including complete blood picture, random blood sugar, and liver and kidney functions

• Revision of their CT studies of the chest

• MRI examination of the chest including diffusion sequences

• Histopathological assessment

MRI protocol

MRI was performed on 1.5 Tesla closed magnet unit (Achieva; Philips Medical Systems, Best, The Netherlands) using a 16-channel phased-array torso coil (Sense XL Torso; Philips Healthcare). To obtain the highest quality MRI, infants and children were given sedation medications through an intravenous (IV) line by a specialized anesthetist. Respiratory gating has been used. The MR scanning sequences are T1W1, T2WI, T2 STIR and DWI, ADC map, and quantitative DWI analysis (ADC measurement).

• T1WI was obtained with the spin-echo sequence with the following parameters: repetition time/echo time, 10 ms/5 ms; number of excitations, 2; direction of frequency encoding, R/L; section thickness, 8 mm; gap, 0.5 mm; field of view, 36–40 cm; matrix, 288 × 224.

• T2WI was obtained with the following parameters: repetition time/echo time, 664 ms/80 ms; number of excitations, 3; direction of frequency encoding, R/L; section thickness, 8 mm; gap, 1.5 mm; field of view, 36-40 cm; matrix, 288 × 224.

• T2 STIR was obtained with the following parameters: repetition time/echo time, 1.6 ms/20 ms; number of excitations, 3; direction of frequency encoding, R/L; section thickness, 10 mm; gap, 1 mm; field of view, 36-40 cm; matrix, 288 × 224.

• DWI is typically acquired in a transverse plane, using three b values; low (0–50 s/mm²), intermediate (500 s/mm²), and high b value (1000 s/mm²). The typical slice thickness is 4–9 mm with an interslice gap of 0–1.5 mm, and the number of excitations ranges from 1 to 10.

• ADC map images and quantitative DWI analysis (ADC measurement).

MR data evaluation and analysis

Lesion assessment

Qualitative assessment

Each lesion was evaluated for its location, extent, size, and relation to adjacent structures. Its signal intensity was evaluated in all pulse sequences T1 WI, T2 WI, STIR WI, and its signal in DWI and ADC maps were compared.

Quantitative assessment

ADCs values were calculated from the ADC maps which were constructed from b = 0 and b = 1000 s/mm² values. A ROI (region of interest) was drawn within areas visually judged to be the most restricted, and the size of ROI was kept as large as possible on the ADC map avoiding the macroscopic necrosis and major blood vessel in the light of the conventional images. The average of three measurements was recorded as the final result.

Associated findings

The following findings were evaluated in MRI, DWI, and ADC map:

• Presence of hilar or mediastinal lymphadenopathy. In the assessment of lymph nodes, only lymph nodes appropriate to criteria were measured for ADC value. Lesions containing large amounts (more than 50% of total size) of necrosis or calcification were excluded.

Other associated MRI findings were taken into consideration:

• Pleural or pericardial collections

• Presence of other chest lesions as chest wall lesions or breast masses

• Presence of upper abdominal lesions

Histopathological diagnosis

The choice of method of tissue sampling/biopsy depends upon both lesion accessibility and patients’ general condition. Tru cut CT-guided core biopsies were done in 29 patients while bronchoscopic biopsies were obtained in 14 patients.

Statistical analysis

ADC was statistically described in terms of mean ± standard deviation (± SD), median, and range. Comparison of ADC between benign and malignant mediastinal masses. Accuracy of ADC in differentiating benign from malignant lesions using the terms sensitivity and specificity. Receiver operator characteristic (ROC) analysis was used to determine the optimum cut off value. P values of less than 0.05 were considered statistically significant. All statistical calculations were done using computer program SPSS (Statistical Package for the Social Science; SPSS Inc., Chicago, IL, USA) release 23.

Results

This study included 44 cases of mediastinal masses: 27 males and 17 females with mean age of 34.8 (range, 1-69 years) (Table 1). There were 6 patients with multiple mediastinal lesions related to the same pathology, so we made our results on the number of patients, not the masses.

Table 1 Description of the studied cases regarding age, sex, and nature of lesions

The study included 34 malignant cases (77.3%) and 10 benign cases (22.7%). The pathological diagnosis, their number/percentage, and corresponding mean ADC value are shown in Table 2.

Table 2 Pathological findings and their mean ADC values

Most of the cases (38 cases) have a single mediastinal lesion which represents 86.4% with majority of lesions (15 cases) are located in the anterior mediastinum which represents 34.1%. The description of the studied cases regarding site in mediastinum, lesions’ dimensions, and number of lesions are presented in Table 3.

Table 3 Description of the studied cases regarding site in mediastinum, lesions’ dimensions, and number of lesions

Regarding the MRI signal intensity, in 41 cases, the masses showed heterogeneous signal intensity representing 93.2% while in 3 cases, the masses showed homogenous signal intensity representing 6.8%. There were 12 cases that showed an isointense signal in DWI (facilitated diffusion) representing 27.3%, while 32 cases showed high signal (restricted diffusion) representing 72.7%. There was a low signal in the ADC map in 35 cases representing 79.5%, while 8 cases were with mixed signal representing 18.2% and only one case with a high signal representing 2.3% (Table 4).

Table 4 Signal intensity of the studied cases by MR sequences, DW, and ADC

There was no statistically significant difference found between the malignant and benign lesions regarding the number of lesions, their site, and lesion signal, while there was a highly significant difference regarding ADC map and DWI. One hundred percent of the benign lesions showed isointense signal in DWI (facilitated diffusion), with 80% showed mixed signal intensity with ADC map, while 94% of malignant lesions showed high signal in DWI (restricted diffusion), with 97.1% showed low signal in ADC map (Table 5).

Table 5 Comparison between benign and malignant tumors regarding signal homogeneity or heterogeneity, DWI, ADC map, and ADC value

The mean ADC value for all cases was 0.98 ± 0.40 × 10⁻³ mm²/s. The mean ADC value for malignant lesions was 0.86 ± 0.35 × 10⁻³ mm²/s, while the mean ADC for benign lesions was 1.39 ± 0.26 × 10⁻³ mm²/s. A comparison between the mean ADC value of the benign and malignant mediastinal lesions was highly significant with P value < 0.001 (Table 5).

The ROC curve (Fig. 2) shows that the best cut off point to predict malignant tumors was found ≤ 1.11 with a sensitivity of 90.9% and specificity of 100% with 100% positive predictive value and 76.9% negative predictive value.

Fig. 2

Receiver operating characteristic curve (ROC) for the predictive value of ADC in differentiation between benign and malignant tumors

MRI findings in cases pathologically proven as bronchogenic carcinoma

This group included 10/44 patients diagnosed as bronchogenic carcinoma (22.8%) (Fig. 3). Six male and four female patients with mean age 55.20 ± 12.59 years (range, 32-69 years). Nine cases showed soft tissue mass lesions with restricted diffusion evident by high signal intensity in DWI, low signal intensity in ADC map, and low ADC values, whereas only one case showed an isointense signal in DWI. The cases showed ADC value ranging from 0.56 up to 0.99 (mean ADC 0.79 ± 0.14) × 10⁻³ mm²/s. Quantitative analysis has also been used to assess associated mediastinal/hilar lymph nodes. Four out of ten patients (40%) had associated lymphadenopathy. Lymph nodes were localized and defined as follows: 2 prevascular, 2 right hilar, 1 paraaortic, 1 subaortic, 1 right paraesophageal, 1 right upper paratracheal, and 1 right lower pre tracheal. All lymph nodes assessed showed restricted diffusion with mean ADC value 0.70 ± 0.26 × 10⁻³ mm²/s, (range 0.43-1.10).

Fig. 3

Case of undifferentiated small cell lung cancer. A 69-year-old male presented with chest pain. MRI chest (a) axial T1WI, (b) axialT2WI, (c) DWI, (d) ADC map showing right side mediastinal soft tissue mass eliciting low signal in T1WI, high signal in T2WI and showing restricted diffusion (high signal on DWI and low signal on ADC map) with ADC value 0.7 × 10⁻³ mm²/s

MRI findings in cases with hilar/mediastinal lymphadenopathy

Our study involved the evaluation of 8 patients with mediastinal/hilar lymphadenopathy; 7 male patients pathologically proven as lymphoma with age range 14-63 years (Fig. 4). The remaining one was a female patient 60 years old diagnosed as cancer breast with mediastinal lymphadenopathy.

Fig. 4

Case of non-Hodgkin lymphoma. A 14-year-old male patient presenting with fatigue and loss of weight. MRI chest (a) axial T2WI, (b) axial DWI, (c) ADC map showing anterior mediastinal prevascular, right and left paratracheal amalgamated lymph nodes eliciting intermediate signal on T2 WI, patchy high signal on DWI, and low signal on ADC map (restricted diffusion). The mean ADC value was about 0.6 × 10⁻³ mm²/s

Lymph nodes in lymphoma cases localized and defined as follows: 5 amalgamated anterior mediastinal masses, 1 right hilar, 1 right paratracheal, 1 right prevascular, and 1 left prevascular. Lymph nodes in lymphoma showed significantly increased signal in DWI and significant low signal in the ADC map, except for one case that showed a faint high signal in DWI and high signal in ADC map due to necrosis. The mean ADC for lymphoma was (0.84 ± 0.43) × 10⁻³ mm²/s, with range 0.50-1.80. Associated MRI findings were seen in 2 cases included pleural effusion in 1 case and 2 chest wall masses in 1 case.

Lymph nodes in breast cancer cases were localized as follows: right and left prevascular, right paratracheal, right paraesophageal, and retro-sternal. They showed a significantly increased signal in DWI and a significant low signal in the ADC map.

MRI findings in cases pathologically proven as neurogenic tumor

This study included 6 cases, three males and three females with age range 30-52 years diagnosed as neurogenic tumor, pathologically proven as follows: 2 cases neurofibroma, 2 cases ganglioneuroma, 1 case malignant schwannoma, and 1 case ganglioneuroblastoma.

Malignant schwannoma and ganglio-neuroblastoma showed high signal (restricted diffusion) in DWI and low signal in the ADC map with low ADC values. Ganglioneuroma case showed an isointense signal in DWI and mixed signals in the ADC map. Neurofibroma cases showed an isointense signal in DWI and patchy low signals in the ADC map. The low ADC signal likely attributed to that the neurofibromas are cellular tumors that show a mixture of Schwann cells, neuronal axons, fibroblasts, mast cells, and collagen. All neurogenic tumor cases showed ADC values ranging from 0.55 to 1.49, with mean ADC (1.08 ± 0.32) × 10⁻³ mm²/s. In the malignant neurogenic tumors, the ADC value was < 1.1 × 10⁻³ mm²/s; while in the benign neurogenic tumors, it was ADC > 1.3 × 10⁻³ mm²/s.

MRI findings in cases pathologically proven as thymic origin

This study included 5 cases, three females and two males with age range 22-60 years. They were pathologically diagnosed as follows: 1 case of thymoma, 2 cases thymic cancer, 1 case of prominent thymus, and 1case of mature thymic tissue. Thymic cancer and thymoma cases showed restricted diffusion in DWI, and low signal in the ADC map, with low ADC values. In this study, the mean ADC value for thymic tumors was 1.08 ± 0.32 × 10⁻³ mm²/s.

Other cases

• 3 cases of germ cell tumor with age range 1-17 years, were pathologically diagnosed as follows:

Seminoma (2 cases), showing restricted diffusion with DWI, and low signal with ADC map, with low ADC values.

Teratoma (1 case), showing a faint high signal with DWI, and variable signals with ADC map, with higher ADC value (Fig. 5).

• 2 cases of liposarcoma (Fig. 6) having age 60 and 68 years presented with heterogeneous mediastinal masses, showing restricted diffusion with DWI, and low signal with ADC map, with low ADC values.

• 2 cases of undifferentiated carcinoma having age 55 and 66 years showing heterogeneous mediastinal masses, with restricted diffusion with DWI, and low signal with ADC map, with low ADC values (0.9 × 10⁻³ mm²/s).

• 2 cases of neuroendocrinal tumor, one of them neuroendocrinal carcinoma, having age 66 years (Fig. 7) showing restricted diffusion with DWI, and low signal with ADC map, with low ADC value (0.9 × 10⁻³ mm²/s). The second case is a carcinoid tumor having age 57 years showing faint high signal with DWI, and mixed signals in ADC map with ADC value (1.5 × 10⁻³ mm²/s).

• One case of the following:

• Bronchogenic cyst having age 32 years presented with homogeneous posterior mediastinal mass, showing a faint high signal with DWI, and variable signal with ADC map, with ADC value (1.7 × 10⁻³ mm²/s).

• Paracardiac cyst having age 40 years presented with homogeneous middle mediastinal mass, showing faint high signal with DWI, and variable signal with ADC map, with ADC value (1.8 × 10⁻³ mm²/s).

• Rhabdomyosarcoma having age 11 years presented with heterogeneous posterior mediastinal mass, showing restricted diffusion with DWI, and low signal with ADC map, with low ADC value (0.5 × 10⁻³ mm²/s).

• Synovial sarcoma having age 25 years presented with heterogeneous middle mediastinal mass, showing restricted diffusion with DWI, and low signal with ADC map, with low ADC value (0.9 × 10⁻³ mm²/s).

• Metastatic renal cell carcinoma having age 42 years presented with heterogeneous anterior mediastinal mass, showing restricted diffusion with DWI, and low signal with ADC map.

• Only one case could not be pathologically diagnosed, but it was presented with heterogeneous anterior mediastinal mass, showing restricted diffusion with DWI, and low signal with ADC map, with low ADC value (0.7 × 10⁻³ mm²/s).

Fig. 5

Case of teratoma. A 14-year-old male patient presenting with dyspnea. (a) axial T1WI, (b) axial T2WI, (c) DWI, (d) ADC map showing mediastinal soft tissue mass showing heterogeneous signal intensity in T1 and T2WI, isointense to slightly faint high signal in DWI and mixed signals in ADC map. The mean ADC is 1.3 × 10⁻³ mm²/s

Fig. 6

Case of liposarcoma. A 60-year-old male patient presenting with progressive loss of weight, chest pain, and dyspnea. MRI chest (a) axial STIR, (b) axial T2, (c) DWI, and (d) ADC map: showing large posterior mediastinal mass of hyper intense signal on T2 WI, intermediate signal on STIR with patchy hyperintense areas on DWI, and low signal on ADC map (restricted diffusion). The mean ADC value was 0.5 × 10⁻³ mm²/s. Bilateral minimal pleural effusion is noted

Fig. 7

Case of neuroendocrinal carcinoma. A 66-year-old female patient complaining of chest pain and weight loss. MRI chest (a) axial T2WI, (b) DWI, and (c) ADC map showing anterior mediastinal mass being hypointense on T2 WI, showing restricted diffusion (high signal on DWI and low signal on ADC map) with a mean ADC value of 0.92 × 10⁻³ mm²/s

Discussion

Depending only on the qualitative assessment of diffusion images to differentiate between benign and malignant masses is not sufficient, because some lesions can show atypical imaging features [8]. We had two malignant lesions with facilitated diffusion patterns; they were proved to be bronchogenic carcinoma and lymphoma. These false-negative results likely were attributed to presence of necrosis. In the study of El-Nahas M. et al. [8], there were three pathologically proved benign lesions with restricted diffusion pattern and two malignant lesions with facilitated diffusion pattern.

So in addition to the data obtained from conventional MRI, DWI enabled us to obtain more information derived from the microscopic motion of water protons. Also, quantitative tumor assessment is possible by the calculation of ADC, which is related to the proportion of extracellular and intracellular components [9].

The difference in the ADC value between malignant and benign tumors is explained by the difference in the histopathological features of benign and malignant tumors. Malignant tumors show hypercellularity and have enlarged nuclei, hyperchromatism, and angulation of nuclear contour. These reduce the extracellular matrix and the diffusion space of water protons in the extracellular and intracellular dimensions with a resultant decrease in ADCs [10].

The study included 34 malignant cases (77.3%) and 10 benign cases (22.7%). We found that the mean ADC value of malignant mediastinal lesions was significantly lower than the mean ADC value of benign mediastinal lesions, with mean ADC 1.39 ± 0.26 in benign mediastinal lesions and mean ADC 0.86 ± 0.35 in malignant mediastinal lesions. This result agrees with the results of Nasr A. et al. [4], who reported that the mean ADC value for the malignant lesions was 0.91 ± 0.23 × 10⁻³ mm²/s while the mean ADC value for the benign lesions was 1.80 ± 0.55 × 10⁻³ mm²/s. This study results are also consistent with the results of El-Nahas M. et al. [8], Dawood H.A., and Salah-Eldin M [9]., Abdel Razek A [11]., and Gümüştaş S. et al. [12].

In agreement with the results of Nasr A. et al. [4], Dawood H.A. and Salah-Eldin M., [9], and Tondo et al. [13], the results of our study demonstrated that the cutoff threshold of ADC value for the differentiation between malignant and benign lesion was 1.11 × 10⁻³ mm²/s, with an area under ROC curve of 0.93. The sensitivity and specificity of our cutoff ADC values were 90.9% and 100%, with 100% positive predictive value and 76.9% negative predictive value. However, Abdel Razek A [11]., Gümüştaş et al. [12], and Sabri Y. et al. [14] reported higher cutoff ADC values of 1.56 × 10⁻³ mm²/s, 1.39 × 10⁻³ mm²/s, and 1.4 × 10^-3 mm²/s, respectively, for the discrimination between malignant and benign mediastinal lesions. This variation of cutoff ADC values is likely attributed to the discrepancy in the sample size and the MRI technique specially the use of different b values.

The results of our study did not find a significant difference in the mean ADC value between lymphoma and other malignant tumors. This is concordant with the results of Nasr A. et al. [4], Gümüştaş et al. [12], and Abdel Razek A. et al. [10] who compared ADCs of lymphomas and bronchogenic carcinomas and there was no significant difference in their mean ADCs. But these results are discordant with the results of Sumi M. et al. [15], who found a significant difference in the mean ADC value between lymphoma and carcinoma. Also, Nomori et al. [16] reported that the ADCs of lymphomas were significantly smaller than those of carcinomas.

Our study involved the evaluation of 12 patients with malignant mediastinal/hilar lymphadenopathy in cases of central bronchogenic carcinoma, lymphoma, and metastatic breast cancer, which showed restricted diffusion, showing low ADC value with mean ADC value (0.79+/−0.32) × 10⁻³ mm²/s (range 0.43-1.8). This is concordant with the results of Abdel Razek A [11]., who reported that the metastatic lymph nodes are usually hypointense on the calculated ADC map with low ADC value (1.01+/−0.02 × 10⁻³ mm²/s). These are also similar to the results of Ustabasioglu F.E. et al. [17], Nakayama J. et al. [18], and Kosucu P. et al. [19], who reported that the mean ADC value in malignant lymph nodes is low, with mean (1.030 ± 0.245 × 10⁻³ mm²/s), (0.24 × 10⁻³ mm²/s), and (1.012+/−0.025 × 10⁻³ mm²/s) respectively.

Mediastinal lymphadenopathy that detected in our cases of central bronchogenic carcinoma, showed restricted diffusion with mean ADC value 0.70 ± 0.26 × 10⁻³ mm²/s (range 0.43-1.10). In the study of Hasegawa et al. [20], who found that diffusion MR imaging can be used for the diagnosis of metastatic lymph nodes in patients with non-small cell lung carcinoma (NSCLC) with a high degree of accuracy (95%).Whereas Nomori et al. [16] reported that the cutoff ADC value used to differentiate metastatic from non-metastatic lymph nodes in patients with NSCLC is 1.63 × 10⁻³ mm²/s, with an accuracy of 89%.

The mean ADC value in lymphoma cases in this study was 0.84 ± 0.43 × 10⁻³ mm²/s (range 0.50-1.80). Most lymphomas are hypercellular, with enlarged nuclei and hyperchromatism, which result in a contracted extracellular space and decreased ADC. However, the ADC may be misleadingly elevated depending on differences between lymphoma types in cellularity, intracellular architecture, the presence and amount of necrosis, and perfusion [5]. This may explain why there is one case that showed elevated ADC value 1.8 × 10⁻³ mm²/s with faint high signal in DWI and high signal in the ADC map. The case was non-Hodgkin lymphoma. Sumi et al. [15] and Sabri et al. [2] reported that the mean ADC value of lymphoma was (0.454 ± 0.075 × 10⁻³ mm²/s) and (1.22 ± 0.23) × 10⁻³ mm²/s respectively.

Grading of malignancy and differentiation of low from high-grade malignancy is essential for prognosis. In this study, there were only three undifferentiated malignant cases, when compared to other differentiated malignant cases, we found no significant difference in the mean ADC value between both groups. This is discordant with the results of Abdel Razek A. et al. [10], who stated that there was a significant difference in the ADC value between well differentiated and poorly differentiated malignancy.

In our study, the mean ADC value for the only one adenocarcinoma case was not significantly higher than small cell lung cancer, which is also discordant to Abdel Razek A. et al. [10], who reported adenocarcinoma type of lung cancer revealed a high ADC value (2.1 × 10⁻³ mm²/s) that was mistaken as a benign tumor. Matoba et al. [21] reported that the mean ADC value of adenocarcinoma was significantly higher than that of squamous cell carcinoma and large cell carcinoma (2.1 × 10⁻³ mm²/s vs. 1.6 × 10⁻³ mm²/s vs. 1.3 × 10⁻³ mm²/s, respectively). It was also reported that the mean ADC value of well-differentiated adenocarcinoma was significantly higher than the values for moderately and poorly differentiated squamous cell carcinoma and poorly differentiated adenocarcinoma. This finding is also supported by Mori et al. [22]. On the contrary, Koyama et al. [23] reported that quantitative DWI is less sensitive than STIR in the detection and subtype classification of pulmonary adenocarcinoma. Therefore, DWI should always be evaluated in conjunction with conventional MRI sequences.

The limitations of our study included the relatively small number of cases in general as well as in each pathological entity. Furthermore, the lack of sizeable comparative group using another functional imaging modality as 8F-FDG-PET/CT.

Conclusion

With the advances in MR systems, DWI of the chest has become possible with fast imaging time that reduces the effect of cardiac and respiration movement. DWI is a non-contrast functional MR imaging technique adding in the analysis of tissue characteristics based on the diffusion of water protons within the tissue. Quantitative assessment of a mediastinal mass is done by calculating its ADC value which is inversely correlated with tissue cellularity. So, DWI has diagnostic potential to distinguish benign from malignant tumors because of the tendency of the malignant lesions to show lower ADC values and more restricted diffusion.