Rectal operative bed: ?tumor? post-management changes … positron emission tomography/computed tomography (PET/CT) can answer

Abstract

Background

Rectal cancer is a common malignancy that continues to have a highly variable outcome. Local pelvic recurrence following surgery is usually leading to incurable disease. Surgical resection with stage-appropriate combined-modality therapy is the mainstay in the treatment of rectal cancer. A difficulty has been reported in distinguishing the benign post-management findings from tumor recurrence. By adding the metabolic changes to the post-operative morphologic changes, combined positron emission tomography (PET) and computed tomography (CT) may offer clinically useful addition in operative bed evaluation.

Results

The study included 30 patients, 17 males (56.6%) and 13 females (43.3%). Suspicious operative bed findings included pre-sacral soft tissue density noted in 20 patients (66%), anastomotic site mural thickening in five patients (16.6%), and post-operative loco-regional nodes in five patients (16.6%). Moreover, pre-sacral soft tissue focal avid uptake was noted in 17 patients (17/20, 85%), and no uptake noted in three patients (3/20, 15%). Anastomotic site mural thickening was seen in five patients with positive uptake noted in three patients (3/5, 60%), and no uptake seen in two patients (2/5, 40%). Loco-regional nodes were seen in five patients with positive uptake in five patients. The most accurate SUV max cut-off value among studied cases was 3.5. Confirmation of PET/CT results was done by histopathological assessment in ten patients (33.3%) and by follow up after 6 months in 20 patients (66.6%) with recurrence noted in 15 patients (75%) and normal post-operative findings noted in five patients (25%).

Conclusion

PET/CT study is an effective tool for evaluation of suspicious colorectal oncologic operative bed findings noted in the routine CT studies. PET/CT fusion images affect the clinical management by guiding further procedures (surgery and radiation therapy).

Background

Rectal cancer is a common malignancy that have variable outcome; 30% of colorectal cancer are located in the rectum. Local invasion of the tumor into deeper tissues lead to peritoneal metastasis, whereas its spread through vascular structures and regional drainage lead to liver, lung, and bone marrow metastasis. Rectal cancer can spread to adjacent structures such as adjacent adipose tissue, vagina, prostate, bladder, ureter, and the bony pelvis; there are different surgical approaches for management of operable cancer rectum, for example low anterior resection (LAR) procedure for recto-sigmoid operable malignancies with total mesorectal excision (TME), and abdomino-perineal resection (APR) for ano-rectal operable malignancies [1].

The post-operative field may show spectrum of normal post-operative findings, pre-sacral soft tissue density thickening notably following abdomino-perineal resection, anastomotic site mural thickening, locoregional subcentimeter lymph nodes, and pre-sacral fluid density collections [2, 3].

Unfortunately, recurrent neoplastic activities could be presented with morphologically similar changes especially pre-sacral soft tissue density and anastomotic site mural thickening [4, 5].

Conventional MDCT studies could not be totally conclusive to discriminate between the expected normal post-operative findings and early recurrent neoplastic process with difficult differentiation; moreover, the post-operative findings may persist for years or even indefinitely [5].

Histopathological assessment is the gold standard technique yet the lesions are not always accessible for biopsy; serial follow up is important in differentiation yet this is at the expense of missing the chance of early management and the patients may present with a progressive course or metastatic lesions in the follow up periods; post-operative MRI studies may be limited by the expected post-operative filed potential artifacts like surgical staples [6].

By adding the metabolic changes (expected increase glucose metabolism by the tumor tissues) to the post-operative morphologic changes, combined positron emission tomography (PET) and computed tomography (CT) may offer clinically useful addition in operative bed evaluation and early detection of recurrence by measuring the degree of accumulation of the fluorodeoxyglucose in the malignant cells helping to make differentiation [6,7,8].

Methods

This is a prospective analysis of 30 patients with pathologically proven rectal cancer managed by surgery with or without adjuvant radiotherapy with suspicious operative bed findings noted in CT colonography study done for all patients.

Suspicious CT data for malignancy then evaluated using PET/CT scanner, PET/CT was done at least 6 weeks after the surgical intervention or radiotherapy, using visual assessment method and semi-quantitative analysis measuring the standard uptake value (SUV) max and the normal would be the normal blood pool liver SUV max.

Reference standard

Ten patient lesions were subjected to a biopsy; serial follow up after 6 months was done in 20 patients as the standard references.

Technique

A hybrid PET/CT scanner (Siemens Biograph 64 PET/CT scanner) was used in this study. Before the examination by 6 h, the patients were instructed to fast, except for water. Avoidance of (extreme) exercise for at least 6 h before the study was asked to minimize FDG uptake in muscles and to reduce the false positive results. The allowable blood glucose level for the study was of < 150 mg/dL. Voiding of urine was done before injection, and then approximately 5 MBq/kg body weight of FDG was injected. Further, 60–90 min later, the data were acquired [7]. The patients then were kept lying comfortably and were asked not to talk to avoid false positive uptake. A non-contrast-enhanced CT from mid-thigh to the skull was obtained 60 min after the tracer injection. Nuclear medicine consultant and radiology consultant interpreted the data at Siemens work station.

Ethics approval and consent to participate

All the patients included in this study gave written informed consent to publish the data contained within this study. Approval for this study was obtained from the Research Ethics Committee of our medical institute. All study procedures were carried out in accordance with the Declaration of Helsinki regarding research involving human subjects.

Results

The study included 30 patients, classified according the patients’ sex into 17 males (56.6%) and 13 females (43.3%) (Table 1). Pre-operative tumor locations documented by pre-operative CT colonography studies were upper rectal in 11 patients (36.6%), mid rectal in nine patients (30%), and low rectal in ten patients (33.3%). Suspicious operative bed findings in the follow-up imaging studies included pre-sacral soft tissue density is noted in 20 patients (66%), anastomotic site mural thickening in five patients (16.6%), and post-operative loco-regional nodes in five patients (16.6%) (Table 2). Limited characterization based on CT findings with recommendations for serial follow up was documented.

Table 1 Distribution of the studied patients according to different parameters

Table 2 Distribution of the studied patients according to the suspicious rectal operative bed findings

PET/CT studies showed the following:

• i. Patients with pre-sacral soft tissue density (n: 20):

  • A- Focal avid uptake noted in 17 patients (17/20, 85%) (Fig. 1)

  • B- No uptake noted in three patients (3/20, 15%) (Fig. 2).

• ii. Patients with anastomotic site mural thickening (n: 5):

  • A- Positive uptake noted in three patients (3/5, 60%) (Fig. 3)

  • B- No uptake seen in two patients (2/5, 40%).

• iii. Patients with loco-regional nodes in the operative bed (n:5):

Fig. 1

a Post-operative CT study showing pre-sacral soft tissue density tumefaction. b PET/CT fused image with focal FDG uptake within the left para median aspect of the post-operative fibrosis using the visual assessment method. c PET/CT fused image with SUV max of the lesion 6.6 reflecting local recurrence confirmed by histopathological assessment

Fig. 2

a Post-operative CT study showing pre-sacral soft tissue density tumefaction. b PET/CT fused image with no appreciable FDG uptake within the post-operative fibrosis using the visual assessment method. c PET/CT fused image with SUV max of the lesion 2.2 with no suspicious areas of uptake

Fig. 3

a Post-operative CT study showing mild Hatman pouch anastomotic site mural thickening. b PET image with focal C shaped FDG uptake within the posterior aspect of the anastomotic site. c PET/CT fused image with avid uptake by the visual assessment method

Five patients with positive uptake (Fig. 4) in the five patients (Table 3).

Fig. 4

a Post-operative CT study showing left para rectal soft tissue lesion (black arrow) mostly nodal. b PET/CT fused image with avid uptake of the lesion (black arrow)using the visual assessment method. c PET/CT fused image with SUV max of the lesion 10 reflecting high metabolic activity impressive of nodal deposits

Table 3 Distribution of the studied patients according to the degree of FDG uptake by the suspicious rectal operative bed findings

The SUV max cut-off value among studied cases was 3.5, the highest SUV max value was 11, the lowest SUV max value was 1, and the median SUV max was 6. Confirmation of PET/CT results was done by either:

• A- Histopathological assessment in ten patients (33.3%), for accessible lesions for biopsy with positive results for recurrence.

• B- Follow-up after 6 months in 20 patients (66.6%) when no access for biopsy and follow up showed the following:

  • Recurrence noted in 15 patients (n: 15/20) (75%).

  • Normal post-operative findings noted in five patients (n: 5/20) (25%).

Histopathological assessment was done by CT-guided percutaneous approach in the accessible lesions in ten patients with informative results; this constitutes another merit for PET/CT study for proper guiding for successful informative CT-guided biopsy with targeting the metabolically active areas (with positive FDG uptake) and avoiding the morphologically similar related scar/granulation tissues reducing the false negative or non-informative results.

Illustrative cases

• Case 1: 49-year-old male patient with history of cancer rectum managed by anterior resection on serial follow-up (Fig. 1).

• Case2: 53-year-old female patient with history of mid third rectal cancer managed by anterior resection on serial follow up (Fig. 2).

• Case 3: 50-year-old female patient with history of cancer rectum managed by staged rectal surgery with Hartman pouch creation (Fig. 3).

• Case 4: 49-year-old male patient with history of mid third cancer rectum managed by anterior resection, presented in the 3 months follow up by left para rectal soft tissue nodular lesion mostly enlarged discrete node (Fig. 4).

Discussion

The presence of numerous rectal cancer post-operative bed morphological changes offer a great challenge during serial follow-up of the rectal cancer post-operative patients; similar morphological patterns are noted in the normal post-operative fields as well as the evolving residual/recurrent neoplastic process.

Differentiation of post-therapeutic alterations attributable to scarring, inflammation, and necrosis from residual or recurrent tumors poses significant problems for CT [9].

Fibrotic or necrotic tissue that develops inside a tumor mass after treatment cannot be differentiated by CT from a viable malignant tumor, also tumor tissue within the post-operative fibrosis cannot be differentiated as well [2, 10]. In this study, 17 patients had post-operative fibrosis at surgical bed noted at CT study without any suspicion of recurrence, but FDG PET and PET/CT detected recurrent tumoral tissue (Fig. 1).

In this study, focusing on the role of PET/CT in the post-operative rectal bed and ability to differentiate post-operative sequel from tumor recurrence, many studies focus upon rectal cancer metastatic potential; Van Custem et al. [11] focused upon the extra intestinal hepatic deposits diagnosis and management.

Paes et al. [12] also confirmed that the advantages of FDG PET/CT for the restaging are mostly attributed to the detection of FDG-avid, normal-sized lymph nodes.

In agreement—in this study—with Weston et al. [13], the accurate SUV max cutoff value was 3.5, yet there is discrepancy between the SUV max value in the literature, while Van Hoeij et al. [14] reported in their study the best cut off value of 11; Peng et al. [15] reported the best cut off value of 2.5.

Gordin et al. [16] and Filippi et al. [17] stated that PET/CT altered treatment management in more than 50% of the study population, while in this study PET/CT had changed patient management in 25 out of 30 patients (83%) as we only included patients who were disease free after surgical removal of their primary tumor, referred to PET/CT due to a clinical or radiological suspicion of recurrence and this clarifies the discrepancy between our study and others in the impact of PET/CT on management.

The merits of the study are numerous; it can be helpful through metabolic and morphological characterization in determining risk-adapted treatment and long-term patient outcome by providing more accurate information about the rectal operative bed findings. Guidance for accurate biopsy can be helpful by revising the metabolic PET/CT data with reduction of the false negative or non-informative results.

Limitations of our study included the relative small number of studied patients, plus the ionizing radiation exposure risks in the PET/CT studies compared to relatively safer techniques like MRI examination that need further comparative studies assessment. Considerations about cost-effectiveness have not been part of this study but will be necessary, as they will play an increasing role in the near future as the clinical utility of PET/CT will lead to a change in the diagnostic and management strategy of cancer patients [18, 19].

Conclusion

Accurate re-staging of the post-operative rectal cancer patients can be reached by PET/CT assessment of the rectal operative bed as well as its metastatic potential with alteration of the patient management strategies.